Cloning and sequencing of the Escherichia coli chlEN operon involved in molybdopterin biosynthesis-Reference-Cited by-同舟云学术

Cloning and sequencing of the Escherichia coli chlEN operon involved in molybdopterin biosynthesis

Published:1988-09 Issue:9 Volume:170 Page:4097-4102
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Nohno T¹,Kasai Y¹,Saito T¹

Affiliation:

1. Department of Pharmacology, Kawasaki Medical School, Kurashiki, Japan.

Abstract

The nucleotide sequence of a HinPI-HpaII restriction nuclease fragment which complemented a delta chlE strain of Escherichia coli was determined. Two open reading frames were deduced to be the structural genes for ChlE and ChlN proteins, which have molecular weights of 44,067 and 26,719, respectively. Both proteins were required for complementing a chromosomal deletion of the chlE locus. The chlE and chlN genes were transcribed from a common promoter, chlEp, located upstream of chlE. Transcriptional and translational signal sequences were recognized in this region.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.170.9.4097-4102.1988

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