Determination of Poly-β-Hydroxybutyrate and Poly-β-Hydroxyvalerate in Activated Sludge by Gas-Liquid Chromatography

Author:

Comeau Yves1,Hall Kenneth J.1,Oldham William K.1

Affiliation:

1. Environmental Engineering Group, Department of Civil Engineering, University of British Columbia, Vancouver, British Columbia, Canada V6T 1W5

Abstract

A convenient gas-liquid chromatography procedure to quantify poly-β-hydroxybutyrate and poly-β-hydroxyvalerate in activated sludge was developed by combining lyophilization of the samples, purification of the chloroform phase by water reextraction, and the use of capillary columns. With a flame ionization detector the sensitivity was estimated at 10 −5 g/liter.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference19 articles.

1. A rapid gas chromatographic method for determination of poly-,-hydroxybutyric acid in microbial biomass;Braunegg G.;Bio/Technol.,1978

2. Biochemical model for biological enhanced phosphorus removal;Comeau Y.;Water Res.,1986

3. Comeau Y. W. K. Oldham and K. J. Hall. 1987. Dynamics of carbon reserves in biological dephosphatation of wastewater p. 39-56. In Advances in water pollution control. Proceedings of the International Association on Water Pollution Research and Control (IAWPRC) Conference on Biological Phosphate Removal from Wastewater Rome Italy. Pergamon Press Oxford.

4. Phosphorus release and uptake in enhanced biological phosphorus removal from wastewaters;Comeau Y.;J. Water Pollut. Control Fed.,1987

5. The role and regulation of energy reserve polymers in micro-organisms;Dawes E. A.;Adv. Microb. Physiol.,1973

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