Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea-Reference-Cited by-同舟云学术

Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea

Published:1998-04 Issue:4 Volume:64 Page:1580-1583
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Fitzgerald Nancy B.¹,English R. Samuel¹,Lampel Jay S.¹,Vanden Boom Thomas J.¹

Affiliation:

1. Fermentation Microbiology Research and Development, Abbott Laboratories, North Chicago, Illinois 60064

Abstract

ABSTRACT We report the development of an electrotransformation method applicable to all strains of Saccharopolyspora erythraea examined to date. Vegetatively grown mycelia were rendered electrocompetent by subjecting mycelial suspensions to ultrasound pulses. The protocol provides an alternative route for the introduction of DNA into filamentous microorganisms otherwise recalcitrant to transformation techniques.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.64.4.1580-1583.1998

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