Affiliation:
1. Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom
Abstract
ABSTRACT
A method for formation of high-electrical-resistance seals on the
Neurospora crassa
plasma membrane, allowing resolution of single-ion-channel activity by patch clamp electrophysiology, is reported. Laser microsurgery permits access to the hyphal apex without enzymatic cell wall digestion and loss of morphological polarity. Cell wall reformation is delayed by brefeldin. This method can allow full characterization of apical plasma membrane channels, which are implicated in tip growth.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
16 articles.
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