Affiliation:
1. Department of Pediatrics, University of Colorado School of Medicine, Aurora, Colorado, USA
Abstract
ABSTRACT
Propionic acidemia is caused by a deficiency of the enzyme propionyl coenzyme A carboxylase (PCC) located in the mitochondrial matrix. Cell-penetrating peptides, including transactivator of transcription (TAT), offer a potential to deliver a cargo into the mitochondrion. Here, we investigated the delivery of an α
6
β
6
PCC enzyme into mitochondria using the HIV TAT peptide at several levels: into isolated mitochondria, in patient fibroblast cells, and in a mouse model. Results from Western blots and enzyme activity assays confirmed the import of TAT-PCC into mitochondria, as well as into patient fibroblasts, where the colocalization of imported TAT-PCC and mitochondria was also confirmed by confocal fluorescence microscopy. Furthermore, a single-dose intraperitoneal injection into PCC-deficient mice decreased the propionylcarnitine/acetylcarnitine (C3/C2) ratio toward the normal level. These results show that a cell-penetrating peptide can deliver active multimeric enzyme into mitochondria
in vitro
,
in situ
, and
in vivo
and push the size limit of intracellular delivery achieved so far. Our results are promising for other mitochondrion-specific deficiencies.
Funder
American Heart Association
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
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