Virus-Associated Nucleases: Evidence for Endonuclease and Exonuclease Activity in Rabbitpox and Vaccinia Viruses

Author:

Aubertin Anne-Marie1,McAuslan B. R.1

Affiliation:

1. Department of Cell Biology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110

Abstract

The endonucleolytic action of a deoxyribonuclease activity in rabbitpox and vaccinia virus was established by change in sedimentation rate of denatured 3 H-lambda deoxyribonucleic acid substrate. The presence of two deoxyribonuclease activities in pox-virus is confirmed. Exo- and endonuclease activities are unmasked by treatment of purified virus with the detergent Nonidet P-40 and further enhanced by treatment of viral “cores” with trypsin.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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