Encephalomyocarditis virus RNA polymerase preparations, with and without RNA helicase activity-Reference-Cited by-同舟云学术

Encephalomyocarditis virus RNA polymerase preparations, with and without RNA helicase activity

Published:1991-05 Issue:5 Volume:65 Page:2714-2717
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Dmitrieva T M¹,Norkina K B¹,Agol V I¹

Affiliation:

1. A. N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.

Abstract

RNA template- and primer-dependent preparations of RNA polymerase were purified from encephalomyocarditis virus-infected Krebs-2 cells, using a three-step chromatographic procedure. The RNA duplex-unwinding activity of these preparations was investigated by two assays, using a partially double-stranded RNA template (encephalomyocarditis virus RNA annealed with a long segment of antisense transcript). Less purified preparations of the polymerase appeared to be able to efficiently displace, in an ATP-dependent and RNA elongation-dependent reaction, the antisense segment from the template. However, upon a more extensive purification, the unwinding activity of the RNA polymerase preparations was lost.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.65.5.2714-2717.1991

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