Accuracy of Phenotypic and Genotypic Testing for Identification of Streptococcus pneumoniae and Description of Streptococcus pseudopneumoniae sp. nov

Author:

Arbique Judy C.1,Poyart Claire2,Trieu-Cuot Patrick2,Quesne Gilles2,Carvalho Maria da Glória S.3,Steigerwalt Arnold G.3,Morey Roger E.3,Jackson Delois3,Davidson Ross J.1,Facklam Richard R.3

Affiliation:

1. Department of Microbiology, Division of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada

2. Laboratoire Mixte Pasteur-Necker de Recherche sur les Streptocoques et Streptococcies and Unité INSERM U570, Faculté de Médecine Necker-Enfants Malades, Paris, France

3. Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

Abstract

ABSTRACT We have identified an unusual group of viridans group streptococci that resemble Streptococcus pneumoniae . DNA-DNA homology studies suggested that a subset of these isolates represent a novel species that may be included in the S. oralis- S. mitis group of viridans group streptococci. We suggest that this novel species be termed Streptococcus pseudopneumoniae . A combination of phenotypic and genetic reactions allows its identification. S. pseudopneumoniae strains do not have pneumococcal capsules, are resistant to optochin (inhibition zones, less than 14 mm) when they are incubated under an atmosphere of increased CO 2 but are susceptible to optochin (inhibition zones, >14 mm) when they are incubated in ambient atmospheres, are not soluble in bile, and are positive by the GenProbe AccuProbe Pneumococcus test. The bile solubility test is more specific than the optochin test for identification of S. pneumoniae . Genetic tests for pneumolysin ( ply ) and manganese-dependent superoxide dismutase ( sodA ) and identification tests with a commercial probe, AccuProbe Pneumococcus, do not discriminate between the new species and S. pneumoniae .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference67 articles.

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4. Borek, A. P., D. C. Dressel, J. Hussong, and L. R. Peterson. 1997. Evolving clinical problems with Streptococcus pneumoniae: increasing resistance to antimicrobial agents, and failure of traditional optochin identification in Chicago, Illinois, between 1993 and 1996. Diagn. Microbiol. Infect. Dis.29:209-214.

5. Escherichia vulneris : a New Species of Enterobacteriaceae Associated with Human Wounds

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