Author:
Fitzgerald T J,Miller J N,Sykes J A
Abstract
The interaction of Treponema pallidum (Nichols strain) with cultured cells was investigated under aerobic conditions. Cell monolayers derived from rabbit testicular tissue extended the survival of treponemes as indicated by active motility. Large numbers of organisms rapidly attached to cultured cells. Within 3 h, one to twelve actively motile treponemes were attached to 25 to 50% of the cells. In addition, T. pallidum attained intracellularity as early as 30 min after inoculation of the cell monolayers. In sharp contrast, T. phagedenis biotype Reiter and T. denticola did not attach and did not enter cultured cells. Most importantly, intracellular and/or attached T. pallidum retained virulence for at least 24 h. Similar observations of attachment and retention of virulence were detected with ME-180, a cell line derived from a human cervical carcinoma. Preliminary studies with superoxide dismutase indicated that this enzyme prolonged treponemal motility and retention of virulence in the presence of cultured cells. These data provide guidelines for further investigations of in vitro cultivation of T. pallidum.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference18 articles.
1. An electron microscopic study of a syphilitic chancre;Azar H. A.;Arch. Pathol.,1970
2. Essais de culture in vitro du treponeme pale en symbiose avec du tissu testiculaire de lapin;Bessemans A.;Rev. Belge Sci. Med.,1934
3. The virulence of Spirocheta pallida in culture;Gammel J. A.;Arch. Dermatol. Syph.,1931
4. Superoxide dismutases of Escherichia coli; intracellular localization and functions;Gregory E. M.;J. Bacteriol.,1973
5. On the cultivation of Spirochaeta pallida in living tissue media;Kast C. C.;Am. J. Syph.,1933
Cited by
81 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献