Affiliation:
1. Department of Molecular Genetics, Hebrew University Hadassah Medical School, Jerusalem, Israel.
Abstract
The BENr gene of Candida albicans, which confers resistance on susceptible strains of Saccharomyces cerevisiae to six structurally and functionally unrelated drugs, was described recently (R. Ben-Yaacov, S. Knoller, G. Caldwell, J. M. Becker, and Y. Koltin, Antimicrob. Agents Chemother. 38:648-652, 1994). This gene bears similarity to membrane proteins encoding antibiotic resistance in prokaryotes and eukaryotes. The effect of disruption of this gene on viability and drug susceptibility was determined. The results indicate that the gene is not essential but its inactivation leads to susceptibility to three of the four drugs tested. Inactivation of this gene did not increase the susceptibility of the mutant to benomyl, suggesting that C. albicans has other mechanisms of resistance, some of which may be additional efflux pumps that confer resistance to this tubulin-destabilizing agent.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference36 articles.
1. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains;Alani E.;Genetics,1987
2. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Struhl (ed.). 1994. Current protocols in molecular biology on CD-ROM. Fall ed. John Wiley & Sons Inc. New York.
3. Genetics and biochemistry of yeast multidrug resistance;Balzi E.;Biochem. Biophys. Acta,1994
4. Candida albicans gene encoding resistance to benomyl and methotrexate is a multidrug resistance gene;Ben-Yaacov R.;Antimicrob. Agents Chemother.,1994
5. A positive selection for mutants lacking orotidine-5~-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance;Boeke J. D.;Mol. Gen. Genet.,1984
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