Quantitative Real-Time PCR Assay for Detection of Ehrlichia chaffeensis-Reference-Cited by-同舟云学术

Quantitative Real-Time PCR Assay for Detection of Ehrlichia chaffeensis

Published:2003-08 Issue:8 Volume:41 Page:3870-3872
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Loftis Amanda D.¹,Massung Robert F.¹,Levin Michael L.¹

Affiliation:

1. Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

Abstract

ABSTRACT A real-time PCR assay was developed for the detection of Ehrlichia chaffeensis . The assay is species specific and provides quantitative results in the range 10 to 10 10 gene copies. The assay is not inhibited by the presence of tick, human, or mouse DNA and is compatible with high sample throughput. The assay was compared with previously described assays for E. chaffeensis .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.8.3870-3872.2003

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