Genomic Analysis of Mycobacterium tuberculosis Complex Strains Used for Production of Purified Protein Derivative

Author:

Inwald Jacqueline1,Hinds Jason2,Palmer Si1,Dale James1,Butcher Philip D.2,Hewinson R. Glyn1,Gordon Stephen V.1

Affiliation:

1. TB Research Group, Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB

2. Bacterial Microarray Group, Department of Cellular and Molecular Medicine, St. George's Hospital Medical School, London SW17 0RE, United Kingdom

Abstract

ABSTRACT The genomes of the tuberculin production strains Mycobacterium bovis AN5 and Mycobacterium tuberculosis DT were compared to genome-sequenced tubercle bacilli by using DNA microarrays. Neither the AN5 nor DT strain suffered extensive gene deletions during in vitro passage. This suggests that bovine tuberculin made from M. bovis AN5 is suitable to detect infection with presently prevalent M. bovis strains.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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