Close Relationship between G8-Serotype Bovine and Human Rotaviruses Isolated in Nigeria

Author:

Adah Mohammed I.12,Nagashima Shigeo1,Wakuda Mitsutaka1,Taniguchi Koki1

Affiliation:

1. Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan

2. Department of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria

Abstract

ABSTRACT A bovine rotavirus, NGRBg8, isolated from the feces of a calf with diarrhea in Nigeria was characterized by reverse transcription-PCR, nucleotide sequence analysis, and Northern blot hybridization. The nucleotide sequence of the VP7 gene of the strain was most closely related to that of a Nigerian human G8-serotype strain, HMG035 (99.9%). The NSP1 gene of strain NGRBg8 is highly related (99.4%) to that of a Thai G8 bovine strain, A5-10. Northern blot hybridization revealed a high overall genomic relatedness of bovine strain NGRBg8 with human strain HMG035; all 11 RNA segments hybridized to each other. Thus, the results show the close relationship between G8 bovine and human rotaviruses in Nigeria.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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