Intrawell Absorption of Antisera in a Template Thin-Film Gel Diffusion Method

Author:

Auernheimer Arthur H.1,Zimmerman Robert A.1

Affiliation:

1. Streptococcal Disease Section, Ecological Investigations Program, Center for Disease Control, U.S. Department of Health, Education, and Welfare, Fort Collins, Colorado 80521

Abstract

This report describes a method for in situ absorption of antisera within the template wells used in thin-film microgel diffusion. The intrawell absorption of antisera offers several advantages over previously reported techniques for detecting the presence of given antigens in biological preparations. The procedure is time saving, antigens are conserved, optimum antigen-antibody ratios are easily determined, and tests of several different absorbing antigens can be made on a single plate.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference7 articles.

1. Precipitate patterns in agar gel with sera from human amebiasis and Entamoeba histolytica antigen;Atchley F. O.;J. Parasitol.,1963

2. Modifications of micro agar precipitin test;Auernheimer A. H.;Amer. J. Clin. Pathol.,1962

3. Specific inhibition of precipitation as an aid in antigen analysis with gel diffusion method;Bjorklund B.;Proc. Soc. Exp. Biol. Med.,1952

4. Serological analysis of components in hemopoietic tissue;Bj B.;Proc. Soc. Exp. Biol. Med.,1952

5. The antigenic complex of Streptococcus haemolyticus. I. Demonstration of a type specific substance in extracts of S. haemolyticus;Lancefield R. C.;J. Exp. Med.,1928

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