Improved Microtechnique for the Leptospiral Microscopic Agglutination Test-Reference-Cited by-同舟云学术

Improved Microtechnique for the Leptospiral Microscopic Agglutination Test

Published:1973-06 Issue:6 Volume:25 Page:976-980
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Cole John R.¹²,Sulzer Catherine R.¹²,Pursell Alfred R.¹²

Affiliation:

1. Diagnostic and Research Laboratories, College of Veterinary Medicine, University of Georgia, Tifton, Georgia 31794

2. Leptospirosis Reference Laboratory, Bacterial Immunology Section, Center for Disease Control, Atlanta, Georgia 30333

Abstract

A method for improving the original Galton microtechnique for detecting leptospiral antibodies has been developed. Simultaneous titrations were performed on 281 animal and human sera and 17 hyperimmune sera with the microscopic agglutination (MA) test and the improved microtechnique. Reproducibility of the improved microtechnique was determined independently on 65 animal sera by two laboratory sections. The results obtained by comparing positive test data from human and animal sera indicated that agreement between the original MA test and this new method exceeded 94%, whereas the original Galton microtechnique and the original MA test agreed in a maximum of 77% of the tests. This study indicates that the results obtained with the improved microtechnique are much more comparable to results obtained with the original MA test than are those obtained with the original Galton microtechnique.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.25.6.976-980.1973

Reference5 articles.

1. Galton M. M. R. W. Menges E. B. Shotts Jr. A. J. Nahmias and C. W. Heath Jr. 1962. Leptospirosis. Epidemiology clinical manifestations in man and animals and methods in laboratory diagnosis. Public Health Service Publ. No. 951 Washington D.C.

2. Application of a microtechnique to the agglutination test for leptospiral antibodies;Galton M. M.;Appl. Microbiol.,1965

3. Permanent turbidity standards;Roessler W. G.;Appl. Microbiol.,1967

4. A modified semimicro method for the microscopic agglutination test for leptospirosis;Sulzer C. R.;Health Lab. Sci.,1973

5. Wolff J. W. 1954. The laboratory diagnosis of leptospirosis. Publ. No. 183 American Lecture Series. Charles C Thomas Publisher Springfield mll.

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