A novel Arthrobacter beta-galactosidase with homology to eucaryotic beta-galactosidases

Author:

Gutshall K1,Wang K1,Brenchley J E1

Affiliation:

1. Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA.

Abstract

An Arthrobacter beta-galactosidase has homology with the lysosomal acid beta-galactosidases from humans and mice and with a Xanthomonas manihotis enzyme. Phylogenetic analysis of the deduced amino acid sequence showed an unusual pattern, with this procaryotic enzyme clustering within the animal clade. The gene encodes a subunit of 52 kDa, and the enzyme appears to be active as a dimer. The enzyme hydrolyzed substrates with either a beta-1,4 or a beta-1,3 linkage.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference14 articles.

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4. Kumar S. K. Tamura and M. Nei. 1993. MEGA: molecular evolutionary genetics analysis version 1.01. Pennsylvania State University University Park.

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4;Laemmli U. K.;Nature (London),1970

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