Affiliation:
1. Periodontal Disease Research Center, College of Dentistry, University of Florida, Gainesville 32610.
Abstract
Recently, Tet Q, a tetracycline resistance determinant that confers resistance by a ribosome protection mechanism, was described and added to the two previously described classes, Tet M and Tet O. The first representative of this class, tetA(Q)1, was isolated from Bacteroides thetaiotaomicron DOT. We report the sequencing of a gene isolated from B. fragilis 1126 which also confers tetracycline resistance. Because of its high degree of identity (97%) with the tetA(Q)1 gene, we defined it as tetA(Q)2. MIC studies revealed that tetA(Q)2 provides a low level of resistance to tetracycline when cloned into Escherichia coli. The extensive homology between tetA(Q)1 and tetA(Q)2 supports the idea of a recent horizontal transfer of tet(Q) genes among Bacteroides spp.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference38 articles.
1. Applied Biosystems. 1991. High-quality template DNA for Taq cycle sequencing using DyeDeoxy terminators: an improved preparation procedure p. 1-4. In Applied Biosystems user bulletin vol. 18. Applied Biosystems Foster City Calif.
2. Tetracycline resistance heterogeneity in Enterococcus faecium;Bentorcha F.;Antimicrob. Agents Chemother.,1991
3. Nucleotide sequence of the tet(M) gene of Tn 916;Burdett V.;Nucleic Acids Res.,1990
4. Purification and characterization of Tet(M), a protein that renders ribosomes resistant to tetracycline;Burdett V.;J. Biol. Chem.,1991
5. Identification of chromosomal antibiotic resistance genes in Streptococcus anginosus ("S. millei;Clermont D.;). Antimicrob. Agents Chemother.,1990
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