Detection of Nucleotide Variability in rpoB in both Rifampin-Sensitive and Rifampin-Resistant Strains of Chlamydia trachomatis-Reference-Cited by-同舟云学术

Detection of Nucleotide Variability in rpoB in both Rifampin-Sensitive and Rifampin-Resistant Strains of Chlamydia trachomatis

Published:2003-07 Issue:7 Volume:47 Page:2316-2318
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Dreses-Werringloer Ute¹,Padubrin Ingrid²,Köhler Lars²,Hudson Alan P.¹

Affiliation:

1. Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan

2. Department of Internal Medicine, Division of Rheumatology, Medical School Hannover, Hannover, Germany

Abstract

ABSTRACT A 656-bp PCR fragment from rpoB was sequenced from five rifampin-resistant Chlamydia trachomatis variants selected in vitro from a wild-type parent with a surprising level of genetic variability in this region. Three variants (MIC, 4 μg/ml) showed Ala522→Val in cluster I (codons 507 to 533), which harbors mutations in most rifampin-resistant bacteria. Two high-level resistance variants (MICs, 64 and 256 μg/ml) showed His526→Tyr in cluster I with additional genetic variation, some of which resulted in amino acid substitutions. None of the latter was situated in clusters related to rifampin resistance in other bacteria.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.47.7.2316-2318.2003

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