Complete Genome Sequence of a Little Cherry Virus-2 Isolate from Sweet Cherry in China

Author:

Zhu Dongzi1,Wang Jiawei1,Zong Xiaojuan1,Tan Yue1,Wei Hairong1,Hammond Rosemarie W.2ORCID,Liu Qingzhong13

Affiliation:

1. Scientific Observation and Experiment Station of Fruits in Huang-huai Area, Ministry of Agriculture, Shandong Institute of Pomology, Taian, Shandong, People’s Republic of China

2. U.S. Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Molecular Plant Pathology Laboratory, Beltsville, Maryland, USA

3. Key Laboratory for Fruit Biotechnology Breeding of Shandong Province, Shandong Institute of Pomology, Taian, Shandong, People’s Republic of China

Abstract

The first complete genome sequence of a little cherry virus-2 (LChV-2-TA) isolate from China was determined using small RNA deep sequencing combined with overlapping reverse transcriptase PCR (RT-PCR). Phylogenetic analysis revealed that LChV-2-TA grouped in a well-supported cluster with members of the genus Ampelovirus with close relationships to previously reported LChV-2 isolates.

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

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