Comparison of the Pour, Spread, and Drop Plate Methods for Enumeration of Rhizobium spp. in Inoculants Made from Presterilized Peat

Author:

Hoben H. J.1,Somasegaran P.1

Affiliation:

1. NifTAL Project, Department of Agronomy and Soil Science, College of Tropical Agriculture and Human Resources, University of Hawaii, Honolulu, Hawaii 96822

Abstract

Inoculants prepared with presterilized peat were enumerated by the pour, spread, and drop plate techniques. Results indicated that the three plating methods were interchangeable. The drop plate method was preferred because of its economy in materials and labor.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference4 articles.

1. Comparison of pour and surface plate methods for determination of bacterial counts;Clark D. S.;Can. J. Microbiol.,1967

2. The estimation of the bactericidal power of blood;Miles A. A.;J. Hyg.,1938

3. A manual for the practical study of root-nodule bacteria;Vincent J. M.;IBP (Int. Biol. Programme),1970

4. Effects of storage temperature and enumeration method on Rhizobium spp. numbers in peat inoculants;Wilson D. O.;Trop. Agric. (Trinidad),1980

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