Enhanced Prodigiosin Production in Serratia marcescens JNB5-1 by Introduction of a Polynucleotide Fragment into the pigN 3′ Untranslated Region and Disulfide Bonds into O -Methyl Transferase (PigF)-Reference-Cited by-同舟云学术

Enhanced Prodigiosin Production in Serratia marcescens JNB5-1 by Introduction of a Polynucleotide Fragment into the pigN 3′ Untranslated Region and Disulfide Bonds into O -Methyl Transferase (PigF)

Published:2021-08-26 Issue:18 Volume:87 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Sun Yang¹,Wang Lijun¹,Osire Tolbert¹,Fu Weilai¹,Yi Ganfeng²,Yang Shang-Tian³,Yang Taowei¹,Rao Zhiming¹^ORCID

Affiliation:

1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China

2. Fujian Dabeinong Aquatic Sci. & Tech. Co., Ltd., Zhangzhou, Fujian, China

3. Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio, USA

Abstract

This study highlights a promising strategy to improve mRNA/enzyme stability and to increase production using de novo PNF libraries and the introduction of disulfide bonds into the protein. PNFs could increase the half-life of target gene mRNA and effectively prevent its degradation.

Funder

National Key Research and Development Program of China

National First-Class Discipline Program of Light Industry Technology and Engineering

Program of Introducing Talents of Discipling to Universities

Key research and development program of Ningxia hui autonomous region

Ningxia Hui Autonomous Region's Flexible introduction project

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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