Capped mRNA Degradation Intermediates Accumulate in the Yeast spb8-2 Mutant-Reference-Cited by-同舟云学术

Capped mRNA Degradation Intermediates Accumulate in the Yeast spb8-2 Mutant

Published:1998-09 Issue:9 Volume:18 Page:5062-5072
ISSN:0270-7306
Container-title:Molecular and Cellular Biology
language:en
Short-container-title:Mol Cell Biol

Author:

Boeck Ronald¹,Lapeyre Bruno²,Brown Christine E.¹,Sachs Alan B.¹

Affiliation:

1. Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720,1 and

2. Centre de Recherche de Biochimie Macromoléculaire, CNRS, F-34033 Montpellier, Cedex 1, France2

Abstract

ABSTRACT mRNA in the yeast Saccharomyces cerevisiae is primarily degraded through a pathway that is stimulated by removal of the mRNA cap structure. Here we report that a mutation in the SPB8 ( YJL124c ) gene, initially identified as a suppressor mutation of a poly(A)-binding protein ( PAB1 ) gene deletion, stabilizes the mRNA cap structure. Specifically, we find that the spb8-2 mutation results in the accumulation of capped, poly(A)-deficient mRNAs. The presence of this mutation also allows for the detection of mRNA species trimmed from the 3′ end. These data show that this Sm-like protein family member is involved in the process of mRNA decapping, and they provide an example of 3′-5′ mRNA degradation intermediates in yeast.

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MCB.18.9.5062

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