Mutations Affecting the Rossman Fold of Isoleucyl-tRNA Synthetase Are Correlated with Low-Level Mupirocin Resistance in Staphylococcus aureus-Reference-Cited by-同舟云学术

Mutations Affecting the Rossman Fold of Isoleucyl-tRNA Synthetase Are Correlated with Low-Level Mupirocin Resistance in Staphylococcus aureus

Published:2002-02 Issue:2 Volume:46 Page:438-442
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Antonio Martin¹,McFerran Neil²,Pallen Mark J.¹

Affiliation:

1. Microbial Genomics and Pathogenesis Unit, Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham, BT15 2TT

2. Department of Biochemistry, The Queen's University of Belfast, Belfast, BT12 6BN, United Kingdom

Abstract

ABSTRACT The isoleucyl-tRNA synthetase ( ileS ) gene was sequenced in toto from 9 and in part from 31 Staphylococcus aureus strains with various degrees of susceptibility to mupirocin. All strains for which the mupirocin MIC was greater than 8 μg/ml contained point mutations affecting the Rossman fold via Val-to-Phe changes at either residue 588 (V588F) or residue 631 (V631F). The importance of the V588F mutation was confirmed by an allele-specific PCR survey of 32 additional strains. Additional mutations of uncertain significance were found in residues clustered on the surface of the IleS protein.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.46.2.438-442.2002

Reference18 articles.

1. Chalker, A. F., J. M. Ward, A. P. Fosberry, and J. E. Hodgson. 1994. Analysis and toxic overexpression in Escherichia coli of a staphylococcal gene encoding isoleucyl transfer-RNA synthetase. Gene141:103-108.

2. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem.162:155-159.

3. Cookson, B. D. 1998. The emergence of mupirocin resistance: a challenge to infection control and antibiotic prescribing practice J. Antimicrob. Chemother.41:11-18.

4. Eriani, G., M. Delarue, O. Poch, J. Gangloff, and D. Moras. 1990. Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs. Nature347:203-206.

5. Farmer, T. H., J. Gilbart, and S. W. Elson. 1992. Biochemical basis of mupirocin resistance in strains of Staphylococcus aureus. J. Antimicrob. Chemother.30:587-596.

Cited by 87 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Antimicrobial susceptibilities and genomic characteristics of methicillin‐resistant Staphylococcus aureus resistant to mupirocin in Stockholm, Sweden;APMIS;2023-11-15

2. Staph wars: the antibiotic pipeline strikes back;Microbiology;2023-09-01

3. Cytoplasmic isoleucyl tRNA synthetase as an attractive multistage antimalarial drug target;Science Translational Medicine;2023-03-08

4. Molecular Basis of Non-β-Lactam Antibiotics Resistance in Staphylococcus aureus;Antibiotics;2022-10-08

5. Modification of Biphenolic Anti‐Bacterial to Achieve Broad‐Spectrum Activity;ChemMedChem;2022-02-22