Expression of lux Genes in a Clinical Isolate of Streptococcus pneumoniae : Using Bioluminescence To Monitor Gemifloxacin Activity-Reference-Cited by-同舟云学术

Expression of lux Genes in a Clinical Isolate of Streptococcus pneumoniae : Using Bioluminescence To Monitor Gemifloxacin Activity

Published:2002-02 Issue:2 Volume:46 Page:538-542
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Beard S. J.¹,Salisbury V.¹,Lewis R. J.¹,Sharpe J. A.²,MacGowan A. P.³

Affiliation:

1. Faculty of Applied Sciences, University of the West of England

2. School of Medicine, University of Leicester, Leicester, United Kingdom

3. Bristol Centre for Antimicrobial Research and Evaluation, Southmead Hospital, Bristol

Abstract

ABSTRACT A clinical isolate of Streptococcus pneumoniae was transformed with a plasmid containing the lux operon of Photorhabdus luminescens that had been modified to function in gram-positive bacteria. Cells containing this plasmid produced light stably and constitutively, without compromising the growth rate. Light output was correlated with measurements of optical density and viable counts during exponential growth and provided a sensitive, real-time measure of the pharmacodynamics of the fluoroquinolone gemifloxacin.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.46.2.538-542.2002

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