Culture of Protozoan Parasites

Author:

Visvesvara Govinda S.12,Garcia Lynne S.12

Affiliation:

1. Centers for Disease Control and Prevention, Atlanta, Georgia 30341

2. LSG & Associates, Santa Monica, California 90402

Abstract

SUMMARY The in vitro culture of protozoan parasites involves highly complex procedures, which are subject to many variables. These parasites have very complex life cycles and, depending on the life cycle stage, may require different culture parameters. However, in vitro cultivation is important for many reasons, some of which include: diagnosis, antigen and antibody production, assessment of parasite immune modulating capabilities, drug screening, improvements in chemotherapy, differentiation of clinical isolates, determination of strain differences, vaccine production, development of attenuated strains, and the continued supply of viable organisms for studying host-parasite interactions.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Microbiology (medical),Public Health, Environmental and Occupational Health,General Immunology and Microbiology,Epidemiology

Reference10 articles.

1. Dedet, J. P., F. Pratlong, R. Pradinaud, and B. Moreau. 1999. Delayed culture of Leishmania in skin biopsies. Trans. R. Soc. Trop. Med. Hyg.93:673-674.

2. Diamond, L. S. 1987. Entamoeba,Giardia and Trichomonas, p. 1-28. In A. E. R. Taylor and J. R. Baker (ed.), In vitro methods for parasite cultivation. Academic Press, Orlando, Fla.

3. Evans, D. A. 1987. Leishmania, p. 52-75. In A. E. R. Taylor and J. R. Baker (ed.), In vitro methods for parasite cultivation. Academic Press, Orlando, Fla.

4. Evans, R., J. M. Chatterton, D. Ashburn, A. W. Joss, and D. O. Ho-Yen. 1999. Cell-culture system for continuous production of Toxoplasma gondii tachyzoites. Eur. J. Clin. Microbiol. Infect. Dis.18:879-884.

5. Garcia L. S. 2001. Diagnostic medical parasitology 4th ed. p. 850-872. ASM Press Washington D.C.

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