Affiliation:
1. Viral and Rickettsial Disease Laboratory, State of California Department of Health Services, Richmond, California
2. Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia
Abstract
SUMMARY
The hemoflagellates,
Trypanosoma
spp. and
Leishmania
spp., are causal agents of a number of parasitic diseases having a major impact on humans and domestic animals over vast areas of the globe. Among the diseases are some of the most pernicious and deadly of human afflictions: African sleeping sickness, Chagas' disease, kala-azar, and Oriental sore. The organisms have complex, pleomorphic life cycles typically involving a vertebrate and an invertebrate host, the latter serving as a vector. In the vertebrate host, they are primarily blood and tissue parasites. In their transition from one host to another, the hemoflagellates undergo morphological, physiological, and biochemical changes that facilitate their growth and subsequent transmission. A major goal in the study of the hemoflagellates has been the cultivation in vitro of both vertebrate and invertebrate stages of the organisms. The first types of media used in their cultivation, and still useful for establishment of cultures, were undefined and contained a complex of ingredients. These gave way to semidefined formulations which included tissue culture media as a base and, as a next step, addition of tissue culture cells as a feeder layer to promote parasite growth. More recently developed media are completely defined, having replaced the feeder cells with various supplements. Serum, a sometimes-variable component of the media, can be replaced by various serum substitutes. This review focuses on the hemoflagellates that infect humans, describing stages in the development of media leading to the fully defined formulations that are now available for the cultivation of many of these organisms.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Microbiology (medical),Public Health, Environmental and Occupational Health,General Immunology and Microbiology,Epidemiology
Reference108 articles.
1. Ali, S. A., J. Iqbal, B. Ahmad, and M. Masoom. 1997. A semisynthetic fetal calf serum-free liquid medium for in vitro cultivation of Leishmania promastigotes. Am. J. Trop. Med. Hyg.59:163-165.
2. Armstrong, T. C., and J. L. Patterson. 1994. Cultivation of Leishmania braziliensis in an economical serum-free medium containing human urine. J. Parasitol.80:1030-1032.
3. Avila, J. L., A. Bretana, M. A. Casanova, A. Avila, and F. Rodriquez. 1979. Trypanosoma cruzi: defined medium for continuous cultivation of virulent parasites. Exp. Parasitol.48:27-35.
4. Avila, J. L., R. Perez-Kepp, and A. Bretana. 1983. A minimal medium for the cultivation of infective Trypanosoma cruzi epimastigotes. J. Gen. Microbiol.129:285-291.
5. Baker J. R. 1969. Parasitic protozoa. Hutchinson University Library London United Kingdom.
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