Affiliation:
1. Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany
2. German Centre for Infection Research (DZIF), partner site Bonn-Cologne, Germany
3. Department I of Internal Medicine, University Hospital of Cologne, Cologne, Germany
Abstract
ABSTRACT
For the diagnosis of
Clostridium difficile
infection (CDI), microbiological testing is almost always accomplished through the analysis of stool specimens. We evaluated the performances of rectal swabs with liquid transport medium (FS) and nylon flocked dry swabs for the detection of
C. difficile
. Additionally, the impact on the diagnostic yield of storing swabs at −80°C for up to 3 months was evaluated. Sixty clinical stool samples positive for
C. difficile
by PCR were used for simulating rectal swabbing. FS and dry swabs were dipped into the stool and tested by PCR directly after swabbing at 1 and 3 months after storage at −80°C. Stool and the liquid medium of FS were additionally tested by a combination of glutamate dehydrogenase antigen (GDH) testing and toxin A/B enzyme immunoassay (EIA), as well as by toxigenic culture (TC). Using dry swabs, the PCR-based detection rate of
C. difficile
was equal to the rate using stool samples (30/30 [100%]), whereas the detection rate in FS was significantly lower (25/30 [83.2%];
P
= 0.019). The sensitivities of FS for detecting
C. difficile
by PCR, TC, GDH testing, and toxin A/B EIA were 83.3%, 85.7%, 88%, and 68.9%, respectively. Storage of swabs at −80°C had no impact on the detection rate. FS cannot replace stool samples in the two-step laboratory diagnosis of CDI, as the sensitivities were too low, probably due to diluting effects of the fecal sample in the liquid medium. For simple PCR-based detection of
C. difficile
, dry swabs proved to be a suitable alternative to the use of stool samples.
Funder
Da Volterra, Paris and Innovative Medicines Initiative Joint
Publisher
American Society for Microbiology
Cited by
11 articles.
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