Salmonella Screening Procedure with Tests for β-Galactosidase and Flagellar Antigens

Author:

Wilson Clyde R.1,Padron Adriano P.1,Dockstader W. B.1

Affiliation:

1. Division of Microbiology, Food and Drug Administration, Department of Health, Education, and Welfare, Washington, D.C. 20204

Abstract

Differentiation of Salmonella from other gram-negative bacilli requires several biochemical and serological tests. A simplified 24-hr screening procedure has been devised which allows discarding of large numbers of isolates (picked from selective plating media) before they are subjected to this extensive testing. Cultures of gram-negative organisms isolated to triple sugar-iron slants during routine examination of products for Salmonella were tested for the presence of β-galactosidase and Salmonella flagellar antigens. β-Galactosidase-positive cultures which did not agglutinate in polyvalent flagellar antiserum were considered to be nonsalmonellae. Of 1,103 Salmonella cultures tested, none of the 61 different serotypes was missed by this procedure, whereas 673 (82.3%) of 818 nonsalmonellae were excluded from further testing. This screening procedure eliminates most nonsalmonellae and augments the proportion of cultures undergoing further biochemical and serological testing which will be confirmed as Salmonella.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference13 articles.

1. The ONPG test in diagnostic bacteriology. 2. Comparison of the ONPG test and the conventional lactose fermentation test;Billow P.;Acta Pathol. Microbiol. Scand.,1964

2. Cowan S. T. and K. J. Steel. 1965. Manual for the identification of medical bacteria p. 122. Cambridge Univ. Press New York.

3. Difco Technical Information Manual No. 0168. 1968. Serological identification of the Salmonella p. 7. Difco Laboratories Detroit.

4. Edwards P. R. and W. H. Ewing. 1962. Identification of Enterobacteriaceae 2nd ed. p. 118. Burgess Publishing Co. Minneapolis.

5. Polyvalent Salmonella "H;Hajna A. A.;Pub. Health Rep.,1950

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3