Effectiveness of Different Frankia Cell Types as Inocula for the Actinorhizal Plant Casuarina

Author:

Burleigh Stephen1,Torrey John G.1

Affiliation:

1. Harvard Forest, Harvard University, Petersham, Massachusetts 01366

Abstract

The soil bacterium Frankia of the Actinomycetales , capable of forming N 2 -fixing symbiotic root nodules on a diverse array of actinorhizal plants, has several morphological forms when grown in pure culture. Fresh hydrated preparations of whole cells, hyphae, and spores were all infective on seedlings of Casuarina at different dilutions. Desiccated hyphae showed no infection capacity, while desiccated spores remained infective, although at a reduced level. On the basis of most-probable-number statistics, spore suspensions were 3 orders of magnitude more infective than hyphae.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference11 articles.

1. Alexander M. 1965. Most probable number method for microbial populations p. 815-820. In C. A. Black (ed.) Methods of soil analysis part 1. Chemical and microbiological properties. American Society of Agronomy Madison Wis.

2. An effective strain of Frankia from Casuarina sp;Diem H. G.;Can. J. Bot.,1983

3. Application of statistics to problems in bacteriology;Halvorson H.;J. Bacteriol.,1932

4. Hoagland D. R. and D. I. Arnon. 1950. The water-culture NOTES 2567

5. Lalonde M. and H. E. Calvert. 1979. Production of Frankia hyphae and spores as an infective inoculant for Alnus species p. 95-110. In J. C. Gordon C. T. Wheeler and D. A. Perry (ed.) Symbiotic nitrogen fixation in the management of temperate forests. Forest Research Laboratory Oregon State University Corvallis.

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