Real-Time Reverse Transcription PCR for Detection and Quantitative Analysis of Equine Influenza Virus-Reference-Cited by-同舟云学术

Real-Time Reverse Transcription PCR for Detection and Quantitative Analysis of Equine Influenza Virus

Published:2005-10 Issue:10 Volume:43 Page:5055-5057
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Quinlivan Michelle¹²,Dempsey Eugene³,Ryan Fergus³,Arkins Sean²,Cullinane Ann¹

Affiliation:

1. Virology Unit, Irish Equine Centre, Johnstown, Naas, County Kildare, Ireland

2. Department of Life Sciences, University of Limerick, Limerick, Ireland

3. Department of Biological Sciences, Dublin Institute of Technology, Dublin, Ireland

Abstract

ABSTRACT Equine influenza is a cause of epizootic respiratory disease of the equine. The detection of equine influenza virus using real-time Light Cycler reverse transcription (RT)-PCR technology was evaluated over two influenza seasons with the analysis of 171 samples submitted for viral respiratory disease. Increased sensitivity was found in overall viral detection with this system compared to Directigen Flu A and virus isolation, which were 40% and 23%, respectively, that of the RT-PCR. The assay was also evaluated as a viable replacement for the more traditional methods of quantifying equine influenza virus, 50% egg infectious dose and 50% tissue culture infectious dose. There was a significant positive correlation ( P < 0.05) between the quantitative RT-PCR and both of these assays.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.10.5055-5057.2005

Reference15 articles.

1. Daly, J., T. Blunden, K. Smith, G. Dowd, N. Davis-Poynter, J. Miller, and S. MacRae. 2004. Suspected equine influenza infection in dogs. Office Int. Epizoot. Bull.2004(2):50.

2. de Jong, M. D., V. C. Bach, T. Q. Phan, M. H. Vo, T. T. Tran, B. H. Nguyen, M. Beld, T. P. Le, H. K. Truong, V. V. Nguyen, T. H. Tran, Q. H. Do, and J. Farrar. 2005. Fatal avian influenza A (H5N1) in a child presenting with diarrhea followed by coma. N. Engl. J. Med.352:686-691.

3. Dowdle, W. R., and M. A. Hattwick. 1977. Swine influenza virus infections in humans. J. Infect. Dis.136(Suppl.):S386-389.

4. Detection of Influenza A Viruses from Different Species by PCR Amplification of Conserved Sequences in the Matrix Gene

5. Koopmans, M., R. Fouchier, B. Wilbrink, A. Meijer, G. Natrop, A. D. M. E. Osterhaus, J. E. van Steenbergen, M. du Ry van Beest Holle, M. A. E. Conyn van Spaendonck, and A. Bosman. 2003. Update on human infections with highly pathogenic avian influenza virus A/H7N7 during an outbreak in poultry in the Netherlands. Eurosurveill. Wkly.7:1-4.

Cited by 55 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Evaluation of a novel real-time polymerase chain reaction assay for identifying H3 equine influenza virus in Kazakhstan;Veterinary World;2023-08

2. A Cold Case of Equine Influenza Disentangled with Nanopore Sequencing;Animals;2023-03-24

3. Occurrence of the selected parasites and viral infections in horses and donkeys in Turkey;Medycyna Weterynaryjna;2023

4. An epidemiological overview of the equine influenza epidemic in Great Britain during 2019;Equine Veterinary Journal;2022-08-31

5. Evaluation of targeted next-generation sequencing for detection of equine pathogens in clinical samples;Journal of Veterinary Diagnostic Investigation;2020-12-11