Preclinical Diagnosis of African Swine Fever in Contact-Exposed Swine by a Real-Time PCR Assay-Reference-Cited by-同舟云学术

Preclinical Diagnosis of African Swine Fever in Contact-Exposed Swine by a Real-Time PCR Assay

Published:2005-01 Issue:1 Volume:43 Page:112-119
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Zsak L.¹,Borca M. V.¹,Risatti G. R.¹,Zsak A.¹,French R. A.²,Lu Z.¹,Kutish G. F.¹,Neilan J. G.¹,Callahan J. D.³,Nelson W. M.³,Rock D. L.¹

Affiliation:

1. Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Greenport, New York

2. Department of Pathobiology, University of Connecticut, Storrs, Connecticut

3. Tetracore Inc., Gaithersburg, Maryland

Abstract

ABSTRACT A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.1.112-119.2005

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