Affiliation:
1. Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine, Université de la Méditerranée, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Abstract
ABSTRACT
The shell-vial culture assay is performed routinely in our laboratory. Recently we revisited our experience of using the shell-vial culture assay for the isolation of microorganisms from various clinical samples. Over a 13-year period, we have isolated 580 bacterial strains (5%) from 11,083 clinical samples tested. Over the same period, 285 isolates of rickettsiae, bartonellae, or
Coxiella burnetii
were cultured from a total of 7,102 samples tested. These isolates include 55
Rickettsia
sp. isolates, 95
Coxiella burnetii
isolates, and 135
Bartonella
sp. isolates. Based on our experience with the growth of fastidious microorganisms, we have used a centrifugation shell-vial technique called JNSP, for “je ne sais pas” (“I don't know [what I am growing]”) for the isolation of other microorganisms. A total of 173 isolates were cultured from the 3,861 clinical samples tested using the JNSP method. Of these, 40 isolates had not been grown before on usual axenic medium. These include 2
Staphylococcus aureus
isolates, 7 isolates of
Streptococcus
sp. and related genera, 6
Mycobacterium
sp. isolates, 1
Nocardia asteroides
isolate, 1
Actinomyces
sp. isolate, 1
Brucella melitensis
isolate, 2
Francisella tularensis
isolates, 1
Mycoplasma pneumoniae
isolate, and 1
Legionella pneumophila
isolate. Using this protocol, we have also cultured intracellular bacteria such as
Chlamydia trachomatis
and we have performed the first culture and establishment of
Trophyrema whipplei
. Applied in our laboratory, the shell-vial culture generally exhibits a low rate of success. However, in some cases, this technique allowed microbial diagnosis when classical agar procedure and PCR were negative.
Publisher
American Society for Microbiology
Cited by
79 articles.
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