Development of a Triplex Real-Time PCR Assay for Detection of Panton-Valentine Leukocidin Toxin Genes in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus-Reference-Cited by-同舟云学术

Development of a Triplex Real-Time PCR Assay for Detection of Panton-Valentine Leukocidin Toxin Genes in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

Published:2005-12 Issue:12 Volume:43 Page:6147-6149
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

McDonald Ryan R.¹,Antonishyn Nick A.¹,Hansen Toni¹,Snook Laelie A.¹,Nagle Evelyn¹,Mulvey Michael R.²,Levett Paul N.¹,Horsman Greg B.¹

Affiliation:

1. Provincial Laboratory, Saskatchewan Health, Regina, Saskatchewan, Canada

2. National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada

Abstract

ABSTRACT Community-associated methicillin-resistant Staphylococcus aureus harboring Panton-Valentine leukocidin (PVL) genes is an emerging pathogen. A novel real-time PCR assay for identification of MRSA isolates containing PVL was developed. The PVL assay was used in a triplex format allowing simultaneous amplification of mecA , nuc , and PVL genes in 614 clinical isolates. This assay facilitates the rapid identification of PVL-positive isolates of MRSA.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.12.6147-6149.2005

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