Evaluation of Universal Probes and Primer Sets for Assessing Total Bacterial Load in Clinical Samples: General Implications and Practical Use in Endodontic Antimicrobial Therapy-Reference-Cited by-同舟云学术

Evaluation of Universal Probes and Primer Sets for Assessing Total Bacterial Load in Clinical Samples: General Implications and Practical Use in Endodontic Antimicrobial Therapy

Published:2005-10 Issue:10 Volume:43 Page:5332-5337
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Horz H. P.¹,Vianna M. E.¹²,Gomes B. P. F. A.²,Conrads G.¹

Affiliation:

1. Division of Oral Microbiology and Immunology, Department of Operative and Preventive Dentistry & Periodontology, and Department of Medical Microbiology, RWTH Aachen University Hospital, Aachen, Germany

2. Endodontic Area, Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, SP, Brazil

Abstract

ABSTRACT By reexamining 10 previously published “universal” PCR assays using the ARB phylogenetic software package and database with 41,000 16S rRNA gene sequences, we found that they differed considerably in their coverage of the domain Bacteria . We evaluated the broadest-range real-time quantitative PCR protocol for its efficacy in measuring the antimicrobial effects of endodontic treatments.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.10.5332-5337.2005

Reference33 articles.

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3. Novel Bacterial Lineages at the (Sub)Division Level as Detected by Signature Nucleotide-Targeted Recovery of 16S rRNA Genes from Bulk Soil and Rice Roots of Flooded Rice Microcosms

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5. Gomes, B. P., E. T. Pinheiro, C. R. Gade-Neto, E. L. Sousa, C. C. Ferraz, A. A. Zaia, F. B. Teixeira, and F. J. Souza-Filho. 2004. Microbiological examination of infected dental root canals. Oral Microbiol. Immunol.19:71-76.

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