Tularemia in Denmark: Identification of a Francisella tularensis subsp. holarctica Strain by Real-Time PCR and High-Resolution Typing by Multiple-Locus Variable-Number Tandem Repeat Analysis

Author:

Byström Mona1,Böcher Sidsel2,Magnusson Anna134,Prag Jørgen2,Johansson Anders13

Affiliation:

1. Department of NBC-Analysis, Swedish Defence Research Agency, Umeå, Sweden

2. Department of Clinical Microbiology, Viborg Hospital, Viborg, Denmark

3. Department of Clinical Microbiology, Infectious Diseases, Umeå University, Umeå, Sweden

4. Department of Infectious Diseases, Sundsvall Hospital, Sundsvall, Sweden

Abstract

ABSTRACT We report ulceroglandular tularemia affecting an 8-year-old boy and the first recovery of Francisella tularensis in Denmark. A novel real-time PCR assay was used to identify the strain as F. tularensis subsp. holarctica (type B). Multiple-locus variable-number tandem repeat analysis demonstrated a close genetic relationship to strains from Norway.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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