A New Biocatalyst for Production of Optically Pure Aryl Epoxides by Styrene Monooxygenase from Pseudomonas fluorescens ST

Author:

Di Gennaro Patrizia1,Colmegna Andrea1,Galli Enrica1,Sello Guido2,Pelizzoni Francesca2,Bestetti Giuseppina1

Affiliation:

1. Department of Genetics and Biology of Microorganisms1 and

2. Department of Organic and Industrial Chemistry,2University of Milan, 20133 Milan, Italy

Abstract

ABSTRACT We developed a biocatalyst by cloning the styrene monooxygenase genes ( styA and styB ) from Pseudomonas fluorescens ST responsible for the oxidation of styrene to its corresponding epoxide. Recombinant Escherichia coli was able to oxidize different aryl vinyl and aryl ethenyl compounds to their corresponding optically pure epoxides. The results of bioconversions indicate the broad substrate preference of styrene monooxygenase and its potential for the production of several fine chemicals.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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4. Furuhashi K. Biological routes to optically active epoxides Chirality in industry. Collins A. N. Sheldrake G. N. Crosby J. 1992 167 186 John Wiley & Sons Ltd. Chichester United Kingdom

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