Affiliation:
1. Division of Microbiology, GBF-National Research Centre for Biotechnology, Braunschweig, Germany
Abstract
ABSTRACT
The utilization of 1,2,4,5-tetrachloro-, 1,2,4-trichloro-, the three isomeric dichlorobenzenes and fructose as the sole carbon and energy sources at nanomolar concentrations was studied in batch experiments with
Burkholderia
sp. strain PS14. In liquid culture, all chlorobenzenes were metabolized within 1 h from their initial concentration of 500 nM to below their detection limits of 0.5 nM for 1,2,4,5-tetrachloro- and 1,2,4-trichlorobenzene and 7.5 nM for the three dichlorobenzene isomers, with 63% mineralization of the tetra- and trichloroisomers. Fructose at the same initial concentration was, in contrast, metabolized over a 4-h incubation period down to a residual concentration of approximately 125 nM with 38% mineralization during this time. In soil microcosms,
Burkholderia
sp. strain PS14 metabolized tetrachlorobenzene present at 64.8 ppb and trichlorobenzene present at 54.4 ppb over a 72-h incubation period to below the detection limits of 0.108 and 0.09 ppb, respectively, with approximately 80% mineralization. A high sorptive capacity of
Burkholderia
sp. strain PS14 for 1,2,4,5-tetrachlorobenzene was found at very low cell density. The results demonstrate that
Burkholderia
sp. strain PS14 exhibits a very high affinity for chlorobenzenes at nanomolar concentrations.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference50 articles.
1. Alexander
M.
The soil environment
Introduction to soil microbiology.
Alexander
M.
1977
3
15
John Wiley & Sons
New York N.Y
2. Biodegradation of organic chemicals.;Alexander M.;Environ. Sci. Technol.,1985
3. Microbial bioconcentration of organic pollutants from aquatic systems—a critical review.;Baughman G. L.;Crit. Rev. Microbiol.,1981
4. Beck
U.
Nucleus-chlorinated aromatic hydrocarbons
Ullmann’s encyclopedia of industrial chemistry
5th ed.
Campbell
F. T.
Pfefferkorn
R.
Rounsaville
J. F.
1986
330
355
VCH Verlagsgesellschaft
Weinheim Germany
5. Genetic and biochemical characterization of the broad spectrum chlorobenzene dioxygenase from Burkholderia sp. strain PS12. Dechlorination of 1,2,4,5-tetrachlorobenzene.;Beil S.;Eur. J. Biochem.,1997
Cited by
34 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献