Capsular Typing Method for Streptococcus agalactiae Using Whole-Genome Sequence Data-Reference-Cited by-同舟云学术

Capsular Typing Method for Streptococcus agalactiae Using Whole-Genome Sequence Data

Published:2016-05 Issue:5 Volume:54 Page:1388-1390
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Sheppard Anna E.¹,Vaughan Alison¹,Jones Nicola¹,Turner Paul²³,Turner Claudia²³,Efstratiou Androulla⁴⁵,Patel Darshana⁴,Walker A. Sarah¹,Berkley James A.²⁶,Crook Derrick W.¹,Seale Anna C.²⁶,

Affiliation:

1. Modernising Medical Microbiology Consortium, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom

2. Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom

3. Shoklo Malaria Research Unit, Mae Sot, Thailand

4. Microbiology Reference Division, Public Health England, London, United Kingdom

5. Imperial College, London, United Kingdom

6. KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya

Abstract

ABSTRACT Group B streptococcus (GBS) capsular serotypes are major determinants of virulence and affect potential vaccine coverage. Here we report a whole-genome-sequencing-based method for GBS serotype assignment. This method shows strong agreement (kappa of 0.92) with conventional methods and increased serotype assignment (100%) to all 10 capsular types.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.03142-15

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