Author:
Korch C,Hagblom P,Normark S
Abstract
Neisseria gonorrhoeae 82409/55(pJD1) is postulated to possess six DNA sequence-specific cytosine methyltransferases and one DNA sequence-specific N6-adenine methyltransferase. From the DNA sequencing of the plasmid pJD1 (manuscript in preparation) by a modification of the Maxam and Gilbert chemical cleavage procedure, the cytosine methylation specificities were demonstrated. Five of these methylating enzymes and their respective specificities are M . NgoI (formula; see text) does not methylate the cytosine of its recognition sequence, in agreement with a detected adenine modification. A biological implication of these different DNA methylating activities is discussed.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference18 articles.
1. NgoII, a restriction endonuclease from Neisseria gonorrhoeae;Clanton D. J.;J. Bacteriol.,1979
2. Identification of a new sequence-specific endonuclease, NgoII, from Neisseria gonorrhoeae;Clanton D. J.;J. Bacteriol.,1978
3. A relationship between plasmid structure, structural lability, and sensitivity to site-specific endonuclease in Neisseria gonorrhoeae;Davies J. K.;Mol. Gen. Genet.,1979
4. Elwell L. P. and S. Falkow. 1977. Plasmids of the genus Neisseria p. 137-154. In R. B. Roberts (ed.) The gonococcus. John Wiley & Sons Inc. New York.
5. Restriction and modification in Bacillus subtilis: localization of the methylated nucleotide in the BsuRI recognition sequence;Gunthert U.;Eur. J. Biochem.,1978
Cited by
66 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献