Potential of the Signal Peptide Derived from the <i>PAS_chr3_0030</i> Gene Product for Secretory Expression of Valuable Enzymes in <i>Pichia pastoris</i>-Reference-Cited by-同舟云学术

Potential of the Signal Peptide Derived from the PAS_chr3_0030 Gene Product for Secretory Expression of Valuable Enzymes in Pichia pastoris

Published:2022-05-10 Issue:9 Volume:88 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Shen Qi¹²,Zhou Xiao-Ting¹²,Guo Qian¹²,Xue Yu-Zhen¹²,Xue Ya-Ping¹²^ORCID,Zheng Yu-Guo¹²

Affiliation:

1. Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, People’s Republic of China

2. Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou, People’s Republic of China

Abstract

Although P. pastoris is widely used for the secretory production of pharmaceutical proteins, its successful applications in the secretory production of industrial enzymes are limited. The α-mating factor pre-pro leader is the most widely used secretion signal in P. pastoris , but numerous industrial enzymes cannot be secreted using it.

Funder

Fundamental research funds for provincial universities of Zhejiang

MOST | National Key Research and Development Program of China

Major research program of Zhejiang provincial national natural science foundation of China

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.00296-22

Reference38 articles.

1. Industrial Applications of Enzymes: Recent Advances, Techniques, and Outlooks