Transcriptomic Characterization of Tuberculous Sputum Reveals a Host Warburg Effect and Microbial Cholesterol Catabolism-Reference-Cited by-同舟云学术

Transcriptomic Characterization of Tuberculous Sputum Reveals a Host Warburg Effect and Microbial Cholesterol Catabolism

Published:2021-12-21 Issue:6 Volume:12 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Lai Rachel P. J.¹²³,Cortes Teresa³,Marais Suzaan⁴,Rockwood Neesha⁴²,Burke Melissa L.¹,Garza-Garcia Acely¹,Horswell Stuart¹,Sesay Abdul K.¹,O’Garra Anne¹⁵,Young Douglas B.¹⁶,Wilkinson Robert J.¹⁴²^ORCID

Affiliation:

1. The Francis Crick Institute, London, United Kingdom

2. Department of Infectious Disease, Imperial College London, London, United Kingdom

3. Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom

4. Wellcome Centre for Infectious Diseases Research in Africa, Institute of Infectious Disease and Molecular Medicine and Department of Medicine, University of Cape Town, Cape Town, Republic of South Africa

5. National Heart and Lung Institute, Imperial College London, London, United Kingdom

6. MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, United Kingdom

Abstract

Although a few studies have described the microbiome composition of TB sputa based on 16S ribosomal DNA, these studies did not compare to non-TB samples and the nature of the method does not allow any functional inference. This is the first study to apply such technology using clinical specimens and obtained functional transcriptional data on all three aspects simultaneously.

Funder

Wellcome

European Commission

UKRI | Medical Research Council

Cancer Research UK

Carnegie Corporation of New York

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mBio.01766-21