Visualization of Polydnavirus Sequences in a Parasitoid Wasp Chromosome

Author:

Belle Elise12,Beckage Nancy E.3,Rousselet Jérôme24,Poirié Marylène1,Lemeunier Françoise2,Drezen Jean-Michel1

Affiliation:

1. Institut de Recherche sur la Biologie de l'Insecte CNRS, Faculté des Sciences, 37200 Tours

2. Laboratoire Populations, Génétique et Evolution, CNRS, 91198 Gif-sur-Yvette Cedex

3. Departments of Entomology and Cell Biology and Neuroscience, University of California—Riverside, Riverside, California 92521

4. Station de Zoologie Forestière, INRA, Ardon, 45166 Olivet Cedex, France

Abstract

ABSTRACT Polydnaviruses, obligatorily associated with endoparasitoid wasps, are unique in that their segmented genome is composed of multiple double-stranded DNA circles. We present here the first cytological evidence that virus segments are integrated in the wasp genome, obtained by using in situ hybridization of virus probes with viral sequences in the chromosomes of a wasp from the braconid family of hymenopterans.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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