CRISPR Inhibition of Essential Peptidoglycan Biosynthesis Genes in Mycobacterium abscessus and Its Impact on β-Lactam Susceptibility-Reference-Cited by-同舟云学术

CRISPR Inhibition of Essential Peptidoglycan Biosynthesis Genes in Mycobacterium abscessus and Its Impact on β-Lactam Susceptibility

Published:2022-04-19 Issue:4 Volume:66 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Kurepina Natalia¹,Chen Liang¹²^ORCID,Composto Kaelea¹,Rifat Dalin³,Nuermberger Eric L.³^ORCID,Kreiswirth Barry N.¹²^ORCID

Affiliation:

1. Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, New Jersey, USA

2. Department of Medical Sciences, Hackensack Meridian School of Medicine, Nutley, New Jersey, USA

3. The Center for Tuberculosis Research, Department of Medicine, Johns Hopkins University, Baltimore, Maryland, USA

Abstract

We utilized a CRISPR interference (CRISPRi) assay to control the gene expressions of two predicted essential peptidoglycan biosynthesis genes, pbpB and cwIM , in Mycobacterium abscessus and to evaluate their contribution to β-lactam susceptibility. Our results showed that CRISPR inhibition of each gene led to a significant 3-log 10 reduction in CFU in the presence of imipenem but not for cefoxitin.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/aac.00093-22

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