Early infection of the central nervous system by the GDVII and DA strains of Theiler's virus

Author:

Aubert C1,Brahic M1

Affiliation:

1. Unité des Virus Lents, URA 1157 Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.

Abstract

The DA strain of Theiler's virus, a murine picornavirus, causes a persistent infection of glial cells of the white matter of the spinal cord, associated with chronic inflammation and primary demyelination. The GDVII strain causes an acute fatal grey matter encephalomyelitis. We characterized the target cells of GDVII and DA viruses 4 days following intracerebral inoculation, and we compared the levels of viral RNA within these cells. GDVII virus infected approximately 10 times more cells than DA virus. Whereas GDVII virus infected neurons exclusively, DA virus infected also astrocytes and possible macrophage-microglial cells. The levels of viral RNA in neurons infected with GDVII and DA viruses were of the same order. These results show that DA virus infects glial cells already at the beginning of the disease and that the more efficient spread of GDVII virus is probably not due to a higher level of RNA replication per cell.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference11 articles.

1. Identification of Theiler's virus infected cells in the central nervous system of the mouse during demyelinating disease;Aubert C.;Microb. Pathog.,1987

2. Detection of viral sequences of low reiteration frequency by in situ hybridization;Brahic M.;Proc. Natl. Acad. Sci. USA,1978

3. Simultaneous in situ detection of viral RNA and antigens;Brahic M.;Proc. Natl. Acad. Sci. USA,1984

4. Brahic M. and S. Ozden. 1992. Simultaneous detection of cellular RNA and proteins p. 85-104. In R. G. Wilkinson (ed.) In situ hybridization-a practical approach. IRL Press Oxford.

5. Theiler's virus persists in glial cells during demyelinating disease;Brahic M.;Cell,1981

Cited by 29 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3