Purification and Characterization of Two Epoxide Hydrolases from Corynebacterium sp. Strain N-1074-Reference-Cited by-同舟云学术

Purification and Characterization of Two Epoxide Hydrolases from Corynebacterium sp. Strain N-1074

Published:1994-12 Issue:12 Volume:60 Page:4630-4633
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Nakamura Tetsuji¹,Nagasawa Toru²,Yu Fujio¹,Watanabe Ichiro¹,Yamada Hideaki³

Affiliation:

1. Central Research Laboratory, Nitto Chemical Industry Company Ltd., Tsurumi-ku, Yokohama 230, Japan

2. Department of Applied Biological Sciences, Nagoya University, Chikusa-ku, Nagoya 464, Japan

3. Department of Agricultural Chemistry, Kyoto University, Sakyo-ku, Kyoto 606, Japan

Abstract

Enzymes II a and II b , which catalyze the conversion of epichlorohydrin (ECH) to 3-chloro-1,2-propanediol (MCP), were purified from Corynebacterium sp. strain N-1074, which catalyzes the formation of ( R )-MCP from prochiral 1,3-dichloro-2-propanol via ECH. The specific activity of enzyme II a for the formation of MCP from ECH was about 6.4-fold higher than that of enzyme II b . Both enzymes catalyzed the conversion of 1,2-epoxides to the corresponding diol, although they differed in several enzymatic properties.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.60.12.4630-4633.1994

Reference12 articles.

1. Tartaric acid metabolism. Synthesis with tartrate epoxidase;Allen R. H.;J. Biol. Chem.,1969

2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding;Bradford M. M.;Anal. Biochem.,1976

3. Characterization of the epoxide hydrolase from an epichlorohydrin-degrading Pseudomonas sp;Jacobs M. H. J.;Eur. J. Biochem.,1991

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4;Laemmli U. K.;Nature (London),1970

5. Hydration of 9,10-epoxypalmitic acid by a soluble enzyme from Bacillus megaterium;Michaels B. C.;Biochem. Biophys. Res. Commun.,1980

Cited by 35 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A Career in Biocatalysis: Kurt Faber;ACS Catalysis;2022-03-14

2. Epoxide Hydrolase for the Synthesis of Chiral Drugs;Nanoscience and Biotechnology for Environmental Applications;2019

3. Metabolism of 2-Methylpropene (Isobutylene) by the Aerobic Bacterium Mycobacterium sp. Strain ELW1;Applied and Environmental Microbiology;2015-03-15

4. Synthesis, crystal structure, vibrational spectra, and thermochemical transformations of tris(hydroxymethyl)aminomethane;Russian Journal of Inorganic Chemistry;2014-01

5. QM/MM study of the mechanism of enzymatic limonene 1,2-epoxide hydrolysis;Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics;2012-02