Use of solid-phase PCR for enhanced detection of airborne microorganisms-Reference-Cited by-同舟云学术

Use of solid-phase PCR for enhanced detection of airborne microorganisms

Published:1994-01 Issue:1 Volume:60 Page:374-376
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Alvarez A J¹,Buttner M P¹,Toranzos G A¹,Dvorsky E A¹,Toro A¹,Heikes T B¹,Mertikas-Pifer L E¹,Stetzenbach L D¹

Affiliation:

1. Department of Biology, College of Natural Sciences, University of Puerto Rico, Rio Piedras 00931.

Abstract

The solid-phase PCR (SP-PCR) was compared with a culture-based technique for the detection of aerosolized Escherichia coli DH1. Results with SP-PCR showed an increase in detection sensitivity over that of culture methods. Therefore, SP-PCR may be useful for the detection of airborne microorganisms which may be nonculturable because of aerosolization or sampling stress.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.60.1.374-376.1994

Reference12 articles.

1. Alvarez A. J. G. A. Toranzos M. P. Buttner and L. D. Stetzenbach. 1993. Abstr. Gen. Meet. Am. Soc. Microbiol. 1993 Q366 p. 413.

2. Buttner M. P. and L. D. Stetzenbach. 1991. Evaluation of four 376 NOTES

3. Polymerase chain reaction detection of nonviable bacterial pathogens;Josephson K. L.;Appl. Environ. Microbiol.,1993

4. Membrane bound PCR;Kadokami Y.;Nucleic Acids Res.,1990

5. Precision of the all-glass impinger and the Andersen microbial impactor for air sampling in solid-waste handling facilities;Lembke L. L.;Appl. Environ. Microbiol.,1981

Cited by 88 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Air sampling and ATP bioluminescence for quantitative detection of airborne microbes;Talanta;2024-07

2. Quantitative detection of parasitic ciliate Cryptocaryon irritans in seawater with an optimized sample processing method;Journal of Fish Diseases;2022-02-17

3. Nanobiosensors: A Promising Tool for the Determination of Pathogenic Bacteria;Nanotechnology for Infectious Diseases;2022

4. Recent progress in nanomaterial-based sensing of airborne viral and bacterial pathogens;Environment International;2021-01

5. Methods of Identifying Microbiological Hazards in Indoor Environments;Microbiological Corrosion of Buildings;2020-08-09