Use of solid-phase PCR for enhanced detection of airborne microorganisms-Reference-Cited by-同舟云学术

Use of solid-phase PCR for enhanced detection of airborne microorganisms

Published:1994-01 Issue:1 Volume:60 Page:374-376
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Alvarez A J¹,Buttner M P¹,Toranzos G A¹,Dvorsky E A¹,Toro A¹,Heikes T B¹,Mertikas-Pifer L E¹,Stetzenbach L D¹

Affiliation:

1. Department of Biology, College of Natural Sciences, University of Puerto Rico, Rio Piedras 00931.

Abstract

The solid-phase PCR (SP-PCR) was compared with a culture-based technique for the detection of aerosolized Escherichia coli DH1. Results with SP-PCR showed an increase in detection sensitivity over that of culture methods. Therefore, SP-PCR may be useful for the detection of airborne microorganisms which may be nonculturable because of aerosolization or sampling stress.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.60.1.374-376.1994

Reference12 articles.

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2. Buttner M. P. and L. D. Stetzenbach. 1991. Evaluation of four 376 NOTES

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5. Precision of the all-glass impinger and the Andersen microbial impactor for air sampling in solid-waste handling facilities;Lembke L. L.;Appl. Environ. Microbiol.,1981

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