Alteration of Substrate Specificity and Optimum pH of Sarcosine Oxidase by Random and Site-Directed Mutagenesis-Reference-Cited by-同舟云学术

Alteration of Substrate Specificity and Optimum pH of Sarcosine Oxidase by Random and Site-Directed Mutagenesis

Published:1994-11 Issue:11 Volume:60 Page:4213-4215
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Nishiya Yoshiaki¹,Imanaka Tadayuki²

Affiliation:

1. Tsuruga Institute of Biotechnology, Toyobo Co., Ltd., Tsuruga, Fukui Prefecture 914, Japan

2. Department of Biotechnology, Faculty of Engineering, Osaka University, Yamadaoka, Suita, Osaka 565, Japan

Abstract

The sarcosine oxidase gene was randomly mutated, and F103V, which showed altered specificity, was screened with the plate assay. Five different mutants at position 103 were also constructed. Each mutant exhibited a rather striking change in substrate specificity, except for F103W. The optimum pH was also shifted, depending on the substrate.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.60.11.4213-4215.1994

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