Detection of Salmonella spp. in oysters by PCR

Author:

Bej A K1,Mahbubani M H1,Boyce M J1,Atlas R M1

Affiliation:

1. Department of Biology, University of Alabama at Birmingham 35294.

Abstract

PCR DNA amplification of a region of the himA gene of Salmonella typhimurium specifically detected Salmonella spp. In oysters, 1 to 10 cells of Salmonella spp. were rapidly detected by the PCR following a pre-enrichment step to increase sensitivity and to ensure that detection was based on the presence of viable Salmonella spp.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference31 articles.

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4. Detection of enteric viruses in oysters by using the polymerase chain reaction;Atmer R. L.;Appl. Environ. Microbiol.,1993

5. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. A. Smith J. G. Sideman and K. Struhl (ed.). 1987. Current protocols in molecular biology. John Wiley & Sons Inc. New York.

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