Rapid identification of Staphylococcus aureus in blood cultures by thermonuclease testing

Author:

Madison B M,Baselski V S

Abstract

The detection of thermonuclease activity in 86 blood culture samples containing gram-positive cocci showed 100% correlation with the subsequent identification of the isolate as Staphylococcus aureus by the coagulase test. No positive thermonuclease results were found with 66 samples containing coagulase-negative staphylococci and 56 samples containing other gram-positive organisms. The thermonuclease test provides a rapid, reliable method to identify S. aureus in blood cultures.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference22 articles.

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2. Identification of Staphylococcus aureus by stimultaneous use of tube coagulase and thermonuclease tests;Barry A. L.;AppI. Microbiol.,1973

3. Direct identification of Staphylococcus aureus in blood culture fluid with a commercial latex agglutination test;Doern G. V.;J. Clin. Microbiol.,1982

4. Production and heat stability of staphylococcal nuclease;Erickson A.;Appl. Microbiol.,1973

5. Characterization and identification of coagulase-negative, heat-stable deoxyribonuclease-positive staphylococci;Gramoll J. L.;J. Gen. Microbiol.,1978

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