Reinfection with Chlamydophila abortus by Uterine and Indirect Cohort Routes Reduces Fertility in Cattle Preexposed to Chlamydophila

Abstract

ABSTRACT This study investigated the effects of controlled reinfection on fertility of cattle naturally preexposed to Chlamydophila abortus . All animals had high prechallenge levels of immunoglobulin M (IgM), IgG, IgG1, and IgG2 serum antibodies against ruminant C. abortus in a chemiluminescent enzyme-linked immunosorbent assay. Twenty virgin heifers were estrus synchronized with prostaglandin F2, artificially inseminated 2 to 3 days later, and challenged immediately by intrauterine administration of 0, 10 4 , 10 5 , 10 6 , or 10 8 inclusion-forming units (IFU) of C. abortus . Ten heifers were estrus synchronized, inseminated, and uterine challenged 2 weeks later. These animals were also indirectly exposed to C. abortus infection (cohort challenged) by contact with their previously challenged cohorts. Pregnancy was determined by rectal palpation 42 days after insemination. All anti- C. abortus antibody isotypes increased in heifers following uterine challenge with 10 8 IFU. A total of 11, 83, 50, 66, and 0% of heifers were pregnant after uterine challenge with 0, 10 4 , 10 5 , 10 6 , and 10 8 IFU of C. abortus , respectively. A total of 50 and 65% of heifers were pregnant with and without cohort challenge, respectively. Uterine inoculum dose and cohort challenge (or, alternatively, a negative pregnancy outcome [infertility]) correlated highly significantly with a rise in postchallenge anti- C. abortus IgM levels over prechallenge levels. Logistic regression modeled fertility, with uterine challenge dose and cohort challenge or prechallenge IgM as predictors ( P < 0.05). The models predict that the uterine C. abortus inoculum causing infertility is 8.5-fold higher for heifers without cohort exposure and 17-fold higher for heifers with high IgM levels than for heifers with cohort exposure or with low IgM levels.